Fig 1: Single-cell transcriptome profiling of three adult human APGs(A) Overview of the study design and workflow.(B) Integrated UMAP plot of all 6,589 cells from three APGs with clusters outlined by lineages and cells colored according to the corresponding cell types. LACTO, lactotrope; SOMATO, somatotrope; THYRO, thyrotrope; GONADO, gonadotrope.(C) Integrated UMAP plot of APGs with cells colored according to the expression of classical marker genes of pituitary (PRL, GH1, TSHB, FSHB, POMC, and SOX2).(D) Dot plot of novel marker genes in endocrine cell types of the APG. The color represents the scaled relative expression level of the marker genes in each cell type, and the size indicates the proportion of cells expressing the marker genes.(E) mIHC staining of S100B, SOX2, CLDN4, SOX9, and VIM in sections from an adult APG. Tissues were counterstained with DAPI. The arrowheads indicate S100BPos STEM (SOX2+ S100B+). The arrows indicate S100BNeg STEM (SOX2+ S100B–). Scale bars, 50 µm. See also Figures S1 and S2.